Merry X-mas and happy new year fluorescent bacteria plate art made for Micropia, also Check out the interview with Joachim Goedhart by Sheba, here Credits go to Nika Heijmans (Amsterdam houses, left), Eike Mahlandt (reindeer, right), Sergei Chavez-Abiega (Grinch), Saskia …
The bioactive sphingolipid S1P is present in bloodplasma, bound to carrier proteins, and is involved in many physiological processes, including angiogenesis, inflammatory responses and vascular stabilization. S1P can bind to several G-protein-coupled receptors (GPCRs) activating a number of different signalling …
S1P-mediated G-protein and RhoGTPase signaling to control endothelial barrier function Read more »
After years of trying, biologists have succeeded in creating an extremely bright red fluorescent protein in the lab. This is good news for researchers, including cancer and stem cell researchers, who use fluorescent proteins to track essential cellular processes. The …
Research highlight: Bright red fluorescent protein created Read more »
The small GTPase RhoA is best known for its ability to remodel the actin cytoskeleton of cells. To study the mechanism underlying the spatiotemporal control of RhoA activity by GEFs, single cell imaging was performed with an improved FRET sensor reporting on the nucleotide loading …
Research highlight: Subcellular location of signaling proteins determines cell shape Read more »
The quality of super resolution images obtained by stochastic single-molecule microscopy critically depends on image analysis algorithms. We find that the choice of background estimator is often the most important determinant of reconstruction quality. A variety of techniques have found …
Research highlight: Taming the image background beast Read more »
Annexins, found in most eukaryotic species, are cytosolic proteins that are able to bind negatively-charged phospholipids in a calcium-dependent manner. Annexin A4 (AnxA4) has been implicated in diverse cellular processes, including the regulation of exocytosis and ion-transport; however, its precise …